Department of Chemistry Organizes a Training Course on the Contribution of Analytical Chemistry to the Separation and Quantification of Compounds Using HPLC Technology

The Department of Chemistry at the College of Education for Pure Sciences – University of Samarra organized a training course entitled “The Contribution of Analytical Chemistry to the Separation and Quantification of Compounds Using HPLC Technology.” The course was delivered by Dr. Mohammed Mahmoud Abdullah, Dr. Mahmoud Saad Abdulwahid, and Assistant Lecturer Qaed Bakr.

The course aimed to introduce the HPLC (High-Performance Liquid Chromatography) instrument as one of the most important devices used in analytical chemistry. It is employed to separate, identify, and quantify chemical components with high precision, particularly in pharmaceutical, food, and environmental industries.

The lecturers reviewed the working principle of the instrument, which is based on separating sample components according to differences in their migration speed within the separation column, resulting from variations in their interactions between the mobile phase and the stationary phase.

They also discussed the main components of the device, including the mobile phase reservoir, the high-pressure pump, the injection unit, the separation column, the detector, and the data recording system that displays results in the form of chromatographic peaks, contributing to accurate compound identification and analysis.

The course concluded with a set of recommendations emphasizing the importance of HPLC technology due to its high accuracy and rapid analysis, as well as its ability to analyze complex compounds using small sample quantities, making it an essential tool in drug estimation, environmental pollutant analysis, and the study of biological compounds.

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