Master’s Thesis Discussed in the Department of Biology on Molecular Detection of Some Virulence Factors of Staphylococcus Isolated from Eye Infections

A master’s thesis was discussed in the Department of Biology, College of Education for Pure Sciences – University of Samarra, entitled: “Some virulence factors of Staphylococcus bacteria isolated from eye infections and their inhibition using chamomile plant extracts,” presented by the student (Tharaa Sufyan Nida).

The study aimed to isolate bacterial species belonging to the genus Staphylococcus from eye infections, and to evaluate the sensitivity of the bacterial isolates to commonly used antibiotics in the treatment of eye infections. In addition, a molecular study was conducted on the most antibiotic-resistant isolates using the Polymerase Chain Reaction (PCR) technique to detect virulence genes (mecA, icaA, and tetK). The study also aimed to determine the biological activity of chamomile plant extracts against the isolated bacteria.

The results showed that all samples from patients with eye infections were Gram-positive. Moreover, all isolates exhibited complete resistance to Methicillin, while all were sensitive to Vancomycin. The findings also confirmed that the alcoholic extract of chamomile showed high efficiency in inhibiting bacterial growth, in a concentration-dependent manner, outperforming the aqueous extract, which showed limited effect.

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