Department of Biology Discusses a PhD Thesis on the Efficacy of L-asparaginase Against Human Lymphocytes and Cancer Cell Lines

The Department of Biology at the College of Education for Pure Sciences, University of Samarra, held a public defense for a PhD thesis titled:
“Efficacy of L-asparaginase Produced from E. coli Against Human Lymphocytes and Cancer Cell Lines” presented by the researcher Sweis Younis Hammadi.
Objectives of the Study: The researcher aimed to isolate Escherichia coli from various infections (sepsis) and test its capability to produce the L-ASNase enzyme. The study also included the characterization of the enzyme, determining the impact of pH and temperature on its activity and stability, as well as evaluating the antitumor activity of the E. coli-derived L-ASNase against human lymphocytes.
Key Findings: The results demonstrated that optimizing laboratory culture conditions—including the type of culture media, carbon and nitrogen sources, pH, and incubation period—led to a significant increase in enzyme production. Furthermore, the study indicated that purification steps using Gel Filtration Chromatography effectively enhanced the enzyme’s purity while maintaining
its full biological activity

#College_of_Education_for_Pure_Sciences #University_of_Samarr