The dissertation of the lecturer Swiss Younis receives a “Satisfactory” grade after its defense at the College of Education for Pure Sciences.

The doctoral dissertation of Assistant Professor Swiss Younis Hamadi, a lecturer at the College of Applied Sciences, University of Samarra, was awarded a “Satisfactory” grade after being defended at the College of Education for Pure Sciences at the same university.

The dissertation was titled: “The effectiveness of L-asparaginase produced from E. coli against human lymphocytes and cancer cell lines.”

In her study, the researcher aimed to investigate the antitumor activity of the enzyme L-asparaginase derived from Escherichia coli against human lymphocytes and cancer cell lines, in order to clarify its degree of cellular selectivity. This was achieved by isolating E. coli from different infections and testing its ability to produce the L-asparaginase enzyme, in addition to determining the optimal conditions for its highest production efficiency.

In conclusion, the study found that improving culture conditions such as medium type, carbon and nitrogen sources, pH, and incubation time led to a significant increase in enzyme production. Furthermore, purification steps using gel filtration chromatography contributed to enhancing the purity of the enzyme while maintaining its biological activity.