A master’s thesis at the University of Samarra discusses the histological and physiological effects of metformin on the ovaries of white rats with induced hypothyroidism
A master’s thesis at the College of Education, University of Samarra, titled “Evaluation of the Histological, Physiological, and Molecular Effects of Metformin on the Ovaries of White Rats with Propylthiouracil-Induced Hypothyroidism,” was presented by student Sara Basim Abdul Wahid.
In her thesis, the student aimed to assess several histological, physiological, and molecular parameters in female rats by studying the effects of metformin and propylthiouracil on the histological structure of the ovary and thyroid gland. She also sought to determine the impact of metformin and propylthiouracil on certain thyroid-related hormonal variables, including TSH, T3, and T4, in addition to identifying and analyzing the DNA sequencing of a segment of the leptin gene and detecting any mutations occurring within it.
The histological examination results of the studied organs (ovary and thyroid gland) following metformin treatment showed hyperplasia of the experimental cells and the presence of numerous blood vessels in the ovarian medulla exhibiting hyperemia and infiltration of white blood cells. Additionally, there was a significant decrease in the concentrations of TSH and T3.
On the other hand, the molecular study results indicated the occurrence of heterogeneous mutations and deletion mutations in the leptin gene receptor in the group treated with metformin.
