Ph.D. Dissertation at the University of Samarra Studies the Role of Antioxidants and the Protective Effect of Flavonoids Isolated from Rosemary

ByUniversity of Samarra Translation Office Reading Time: 1 minute

A Ph.D. dissertation in the College of Education at the University of Samarra, titled “The Role of Antioxidants and the Protective Effect of Flavonoids Isolated from Rosemary in Methotrexate-Induced Oxidative Stress in Male Laboratory Rats,” was conducted by researcher Ahmed Jassim Mohammed.

In his dissertation, the researcher meticulously aimed to elucidate the protective effect of flavonoids isolated from the rosemary plant against methotrexate-induced oxidative stress. This was achieved through the qualitative and quantitative estimation of the metabolic compounds present in the plant, the laboratory assessment of the antioxidant strength of the isolated flavonoids, and the evaluation of specific inflammatory cytokines and interleukins in male laboratory rats.

The study’s results indicated that rosemary plant extract contains secondary metabolic compounds such as phenols and flavonoids, among others. The isolated flavonoids contained seven compounds identified by high-performance liquid chromatography (HPLC): p-coumaric acid, apigenin, kaempferol, ferulic acid, quercetin, sinapic acid, and rosmarinic acid. The isolated flavonoids also exhibited a higher DPPH free radical scavenging activity than standard Vitamin C, in addition to possessing good reducing power.

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